In Vitro Pharmacology

Immunomodulation


We offer a comprehensive range of in vitro & ex vivo models for identification of immunostimulant & immunosuppressant potential of molecules

in vitro /ex vivo models for immunomodulators

  • Chemokine /cytokine profiling for immunomodulators ex vivo
    in vitro Model for identification of Immunomodulators
    Model Chemokine & cytokine profiling for identification of immunomodulators
    Test system Murine bone marrow derived dendritic cell cultures
    Methods
    • Generation of Dendritic cells in vitro
    • Characterization of dendritic cells
    • Profiling of potential of test item for modulation of cytokines & chemokines
    • Validation with immunosuppressant & immunostimulatory molecules
    End points Quantitation of TNF- a, IL-1- ß, MIP-1- a , IL-6 ,RANTES, IL -12, IFN– ?, CCL- 22, IP -10, IL -4, IL -10 & TGF-ß
    X
  • Phagocytosis
    in vitro Model for identification of Immunomodulators
    Model Phagocytosis assay for identification of immunostimulants
    Test system Macrophage cell line (RAW 264.7)
    Methods
    • Treatment of macrophages with test compounds
    • Evaluation of immunostimulatory effect of test compounds exhibited by internalization of particulate material (Zymosan) by macrophage cells
    End points Colorimetric estimation of Zymosan uptake by cells
    X
  • NK cell activity
    in vitro Model for identification of Immunomodulators
    Model Natural Killer (NK) cells based assay for identification of immunostimulants
    Test system Effector cells - Murine splenocytes (NK cells) Target cells - Murine lymphoma cell line (YAC-1)
    Methods
    • Treatment of NK cells with test compounds
    • Co-incubation of NK cells with YAC-1 cells
    • Assessment of immunostimulatory effect of test compounds on NK cells mediated lysis of YAC-1 cells
    End points Estimation of NK cells mediated targeted lysis of YAC-1 cells by MTT assay or fluorescence based methods
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