In Vitro Pharmacology

Fertility


  • Screens for Erectile Dysfunction
    Model Post-coital Administration of the test item in female rats
    Parameter
    • Number of implantations
    • Number of corpus lutea
    • Number of Live/Dead Fetuses
    • Anti-fertility Activity
    • Body Weight Change
    End points
    • Estrogen
    • Progesterone
    X
    • PDE5
    • RHO Kinase
  • Assessment of Galactogogue Potential
    Model Evaluation of Galactogogue (Lactogenic) activity
    Test system Mouse mammary epithelium cell line (HC11)
    Method
    • Identification of non-cytotoxic concentrations of Test item by MTT assay.
    • Treatment of cells with Test item at non-cytotoxic concentrations
    • Effect on mammosphere formation by phase contrast microscopy
    • Effect on casein production by western blotting.
    End points
    • No. of mammospheres
    • Casein production
    X