In Vitro Pharmacology

Gastrointestinal


  • Digestive Stimulants
    Model In vitro model for digestive stimulants
    Test system Estimation of digestive enzyme activities using biochemical assays:
    • Pancreatic & intestinal homogenates of rat
    • Commercially available enzymes
    Method Evaluation of test compound’s effect on the activities of digestive enzymes of gastrointestinal tract
    End points
    • Pancreatic amylase, Lipase, Trypsin & Chymotrypsin
    • Intestinal Sucrase, Lactase & Maltase
    Model
    • Cell free biochemical assays
    Test system
    • Pancreatic & intestinal homogenates of wistarrat Commercially available enzymes
    Method Preparation of pancreatic and intestinal homogenates from wistar rat Treatment with test item Pancreatic amylase, Lipase, Trypsin & Chymotrypsin Intestinal Sucrase, Lactase & Maltase
    End points
    • Digestive stimulatory potential of test item reflected by enhanced enzyme activitie
    X
  • Liver Cirrhosis
  • Antiulcer
    Anti-ulcer potential
    • Cel lfree assays–anti-oxidantandant acid
    • Adenocarcinoma cell line(AGS)
    Test system
    • Humana denocarcinoma cell line(AGS)
    Method Anti-oxidant -DPPH/ABTS assay ?Acid neutralizing capacity -Titrimetric assays with NaOH Cytoprotection in AGS cell line -Treatment of AGS cells with test item (5 concentrations) -Exposure of cells to acidic medium (pH-3)/Indomethacin/ethanol -Effect of test item on survival of cell
    End points
    • Anti-ulcer potential of test item reflected by anti-oxidant, antacid and cytoprotective activitie
    X