In Vivo Pharmacology

Oncology


We bring more than 19 years of experience of developing molecules in Oncology. We can design and execute Oncology model of your choice in consultation with you or customize an oncology model specific to your molecules. We provide expertise and services for a variety of classes of molecules viz. cytotoxic, cytostatics, anti angiogenic, small molecules, phytochemicals & signalling modulators. All studies are carried out in a welfare regulations approved animal facility equipped with state-of-the-art barrier facility

  • Xenograft models
    Model Subcutaneous Xenograft Model
    Test system Athymic nude mice or SCID mice
    Models
    • For nSCLC, prostate, ovarian, colon & breast cancers
    • Customized models for other tumorigenic cell lines available
    Method
    • Subcutaneous implantation of human cancer cell lines or tumor fragments
    • Initiation of study at predetermined time points
    • Monitor the tumor volume for growth and body weight for systemic toxicity
    End points
    • Body weight Change
    • Median Tumor volume
    • % Tumor Growth Inhibition
    • Log Cell Kill
    • %T/C
    • Relative Tumor Volume
    • Tumor Growth Delay
    X
  • Syngenic tumour models
    Model Immunocompetent tumour models
    Test system C57BL/6 and BALB/c
    Cell lines Including Melanomas, Lewis lung carcinoma & renal cell lines
    Method
    • Subcutaneous injection of mouse cancer cell line
    • Monitor the growth and randomize with predetermined size
    • Administer the test article for specified period
    • Humane sacrifice and end point capture
    End points
    • Body weight Change
    • Median Tumor volume
    • % Tumor Growth Inhibition
    • Log Cell Kill
    • Growth Delay
    X
  • Orthotopic models
    Model Assessment of anti-cancer activity of the compounds using orthotopic models
    Test system Nude / SCID mice
    Cancer Type
    • For Colon, Pancreas & Breast cancers
    • Customized models for other tumorigenic cell lines available upon client request
    Method
    • Surgical implantation / inoculation of tumor tissue/ cells
    • One week recovery period
    • Administration of test item and reference item
    End points
    • Percentage Change in body weight
    • Survival rate of animals
    • Clinical signs & symptoms
    • Tumor Histopathology
    • Tumor weight
    • Assessment of distant metastasis
    X
  • Angiogenesis models
    Model Matrigel Plug Assay
    Test system C57BL/6
    Models
    • Subcutaneous injection of Matrigel with Growth Factors(bFGF and VEGF)
    • Treatment for specified schedule
    • Humane sacrifice and end point estimations
    End points
    • Matrigel weight
    • Estimation of Hemoglobin content
    • Histopathology
    • Body weight Change
    X
  • Metastasis models
    Model Melanoma Model for Lung Metastasis
    Test system C57BL/6
    Models
    • Intravenous administration of B16F10 melanoma cell line at predetermined cell number
    • Administration of the test article for specified period
    • Humane sacrifice and end point capture
    End points
    • Body weight Change
    • Lung nodule count
    • Melanin content
    • Lymph node mapping
    Model Colon Model for Metastasis
    Test system BALB/c mice
    Models
    • Subcutaneous injection of colon tumor cells (CT-26)
    • Monitoring of tumor growth
    • A dministration of test item and reference item
    • Observation of animals for defined time period
    End points
    • P ercentage Change in body weight
    • Survival
    • Clinical signs & symptomst
    • Median Tumor volume
    • Median Tumor Growth Inhibition
    • %T/C
    • Growth Delay
    • Log Cell Kill
    • Tumor Histopathology
    • Assessment of  distance metastasis
    X
  • Leukemia models
    Model Assessment of anti-leukemic activity of the compounds in SCID mice
    Test system SCID mice
    Models
    • Intravenous inoculation of leukemic cell line
    • Administration of test item and reference item at predetermined time
    • Observation of animals for defined time period
    • Observation of animals in satellite group for occurrence of chloroma
    End points
    • Percentage Change in body weight
    • Survival rate of animals
    • Clinical signs & symptoms
    • Quantification by flow cytometry analysis in bone marrow & peripheral blood
      — Standard surface specific antigen staining - CD45
      — Lineage specific antigen staining
    • Histopathology
    X