- 20µL of TPA solution (containing 2µg of TPA in suitable solvent) will be applied topically on the right ear of mice on day 0,2,4,7 and 9.
- The test item/reference compound will be dissolved in solvent/TPA and applied onto the ear on day1 to 9.
- Ear thickness to be measured everyday.
- 4mm punch biopsy will be taken and weighed on day 10
- Ear tissue will be excised