In Vivo Pharmacology

Invitro Cosmetic Screens


Invivo Cosmetic Screen

  • Screening models for hair growth stimulators
    Model Ex Vivo model for assesing hair growth stimulator
    Test system Mouse vibrissae hair follicle
    Method
    • Isolation of vibrissae hair follicle *
    • Maintenance in growth medium
    • Treatment with test item
    • Incubation period : 15 days – 20 days
    End points
    • Hair shaft length
    • Hair shaft thickness
    • Hair bulb diameter
    • Melanin content
    X
  • In vivo efficacy model to asses hair growth promoters
    Model C3H/HeJ and C57BL/6 mice based models
    Test system Male/Female mice (6-9 weeks of age) in telogen phase of hair growth
    Method
    • Direct relationship between the skin pigmentation and anagen
    • Short and synchronized hair cycle
    • Well characterized hair cycle
    End points
    • Visual melanogenesis
    • Mean hair growth score
    • Percent anagen induction
    • Histological analysis
    • Follicle count in sub cutis
    • Skin thickness
    • Biomarkers
    • Hair Quality
    • Hair thickness
    • Hair weight
    • Hair diameter
    Model C3H or C57BL/6 mouse based model
    Test system Male C3H/HeJ or C57BL/6mice (6-8 weeks of age) in telogen phase of hair grow
    Method
    • Visual melanogenesis
    • Mean hair growth score
    • Percent anagen induction
    • Histological analysis
    • Follicle count in sub cutis
    • Skin thickness
    • Biomarkers
    • Hair Quality
    End points
    • Direct relationship between the skin pigmentation and anagen
    • Short and synchronized hair cycle
    • Well characterized hair cycle
    X
  • Chemotherapy induced Alopecia
    Model In vivo Model for screening molecules that prevent /reduce chemotherapy induced alopecia
    Test system Swiss albino & C57BL/6 models for chemotherapy induced alopecia
    Method
    • Synchronization of hair cycle to Anagen phase
    • Induction of alopecia by Etoposide or Cyclophosphamide
    • Testing of potential of molecule to prevent or reduce alopecia
    End points
    • Hair shaft length
    • Hair shaft thickness
    • Hair bulb diameter
    • Melanin content
    X
  • Screening models for assessment of anti photoageing potential in vivo
    Model In vivo models
    Test system Nude mice
    Method
    • Topical application of test formulation
    • UVB exposure for 7 continuous days
    End points
    • Visual wrinkling
    • Hydration index with Corneometer
    • Erythema by mexamater
    • Histological analysis
    • Dermal thickness
    • Epidermal hyperplasia
    • Inflammation
    • Collagen estimation
    X
  • Hair pigmentation
    Model Hair color pigmentation- Anti-graying/Blackening effect-In vivo model
    Test system Male/Female mice (6-9 weeks of age) in telogen phase of hair growth
    Method
    • Direct relationship between the skin pigmentation and anagen
    • Short and synchronized hair cycle
    • Well characterized hair cycle
    End points
    • Visual melanogenesis
    • Mean hair growth score
    • Percent anagen induction
    • Histological analysis
    • Follicle count in sub cutis
    • Skin thickness
    • Biomarkers
    • Hair Quality
    • Hair thickness
    • Hair weight
    • Hair diameter
    X

Invivo Dermapathology

  • Psoriasis
    Model In-Vivo Psoriasis Model
    Test system BALB/c or C57BL/6 Mice; 7-9week
    Method
    • 20µL of TPA solution (containing 2µg of TPA in suitable solvent) will be applied topically on the right ear of mice on day 0,2,4,7 and 9.
    • The test item/reference compound will be dissolved in solvent/TPA and applied onto the ear on day1 to 9.
    • Ear thickness to be measured everyday.
    • 4mm punch biopsy will be taken and weighed on day 10
    • Ear tissue will be excised
    End points
    • Change in Ear thickness (mm) graph - % Activity
    • Histology - Epidermal ear thickness (in µM) from histology
    • Staining for PCNA positive epidermal cells (IHC)
    • Optional : Pro-inflammatory cytokines (TNF-alpha, IL6 and IL1beta) and MPO activity in serum and ear tissue
    X
  • Acne
    Model SYRIAN GOLDEN HAMSTER EAR ASSAY
    Test system Male Syrian Golden Hamster 7-11 weeks
    Method
    • Application of test formulation by topical route.
    • Inclusion of vehicle control group/positive control groups.
    • Application of 25 µl of test formulation/vehicle/positive control to ~3cm2 of the ventral surfaces of both the right and left ear.
    • Application of test formulation/vehicle/positive control twice daily for 5 days /week for 4 weeks.
    • Collection of ear biopsies from each animal after sacrifice for sebum analysis and histopathological investigations.
    End points
    • Histological analysis for measurement of sebaceous glands size Sebum content analysis of ear samples
    Model RABBIT EAR ASSAY
    Test system Rabbit
    Method
    • Induction of experimental comedones by application of a comedogen (oleic acid/coal tar)
    • Application of test item
    • Visual observation
    • Analysis of skin sections by histology
    • Analysis of skin sections by immunohistochemistry
    End points
    • Ear thickness
    • Number of comedones/papules
    • Histological analysis
    • Epithelial proliferation
    • Sebaceous gland proliferation
    • Infiltration of inflammatory cells
    • Levels of PGE2
    • Expression of Substance P
    X
  • Atopic Dermatitis
    Model Skin injury and Epicutaneous (EC) sensitization with ovalbumin
    Test system Female BALB/c
    Method
    • Skin injury and EC Sensitization with ovalbumin (Three times)
    • Treatment with test compound after third sensitization
    • Collection of serum and skin for end point analysis
    End points
    • Body weight, weight of thymus and spleen
    • Eosinophil count in peripheral blood
    • Serum IL-5, IgE, IgG2a and IFN-? estimation
    • Estimation of IL-5 and IFN- ? in tissue homogenates.
    • Skin Histopathology
    X
  • Wound Healing
    Model Invivo Model for assesment of wound healing potential
    Test system Wistar rat
    Method
    • Creation of 1x1 cm wound on dorsal surface
    • Measurement of wound dimension & randomization
    • Wound will be covered with film spray dressing (Cavilon; 3M)
    • Trimethoprim treatment for 5 days
    • Application of test item as per client defined regimen
    • Wound dimension measurement on days 4, 12 and 21 post wounding
    • Sacrifice of representative animals on day 21 for histopathology
    End points
    • Morphometric analysis of wound closure – wound contraction, percent scar area
    • Histopathology
    • Inflammatory cell infiltration
    • Fibrosis
    • Collagen
    • Fibrin clot
    X