In Vivo Pharmacology

Invivo-others


  • Asthama
    Model OVA induced asthma
    Test system BALBc mice
    Method Randomize and grouping based on body weight Day 1 & 15 : i.p. injection of 50 µg OVA (0.2 ml) complexed with 10 % aluminiun hydroxide (in saline) (Ova sensitisation) Day 21-23 & 28-30 : 2.5 % Ova Nebulisation for 30 min. Administration of test item and reference item (single dose) on 30th day (in case of chronic study 14 day treatment and Ova Nebulisation thrice in a week)
    End points
    • Total cell count in BALF (Bronchoalveolar lavage fluid)
    • Eosinophil Count in BALF
    • Histopathology (only for chronic studies)
    Model OVA induced asthma in BALBc mice
    Test system Balb/c mice
    Method Day 1 & 15 : i.p. injection of 50 µg OVA (0.2 ml) complexed with 10 % aluminiun hydroxide (in saline) (Ova sensitisation) Day 21 up to 8 week : 2.5 % Ova Nebulisation for 30 min.
    End points
    • Histopathology evaluation of lung samples.
    Model OVA induced eosinophilia in Brown Norway rats
    Test system Brown Norway rats
    Method Day 0,1& 2 : i.p. injection of 1 mg of ova albumin + 20 mg of aluminiun hydroxide in 1 ml per animal (in saline) (Ova sensitisation) Day 21 : 1.0 % Ova Nebulisation for 15 min. Day 22 : BALF Collection
    End points
    • Total cell count in BALF
    • Eosinophil Count in BALF
    Model OVA induced eosinophilia in Brown Norway rats
    Test system Brown Norway rats
    Method Day 0,1& 2 : i.p. injection of 1 mg of ova albumin + 20 mg of aluminiun hydroxide in 1 ml per animal (in saline) (Ova sensitisation) Day 21 : 1.0 % Ova Nebulisation for 15 min. Day 22 : BALF Collection
    End points
    • Total cell count in BALF (Brancheo alveolar lavage fluid)
    • Eosinophil Count in BALF (Brancheo alveolar lavage fluid)
    X
  • COPD
    Model LPS induced Neutrophilia/TNFa
    Test system SD rat
    Method
    • Randomize and grouping based on body weight. Administration of test item and reference item 30 minutes prior to LPS exposure 0.5 hr (post treatment) : LPS (in saline) Nebulisation for 40 min 2.0 hr (post treatment) : BALF Collection (for TNFa evaluation) 6.0 hr (post treatment): BALF Collection (for neutrophils evaluation)
    End points
    • Neutrophils count in BALF (Bronchoalveolar lavage fluid)
    • TNFa in BALF
    Model LPS induced Neutrophilia/TNFa
    Test system SD rat
    Method
    • 0.5 hr : LPS (in saline) Nebulisation for 40 min
    • 2.0 hr : BALF Collection
    End points
    • TNFa in BALF
    X
  • LungFibrosis
    Model Bleomycin induced Lung fibrosis
    Test system Sprague- Dawley/ Wistar rat
    Method
    • The animals will be randomized based on body weight After randomization the animals treated with bleomycin by intra tracheal route Administration of test item and reference item at predetermined time (simultaneous treatment for prophylactic and 14 days after intratracheal instillation of bleomycin for therapeutic) Body weight will be recorded on daily basis. At the end of the experiment BALF and lung samples will be collected for further analysis
    End points
    • Total cell count (BALF)
    • Histopathology evaluation (Lung samples)
    X