Dermal Papilla Cells (DPC) proliferation assay by BrdU incorporation to evaluate DPC mitotic activity using Human Follicular Dermal papilla cells (HFDPC), measuring cell proliferation .

• Cell Model

  Human Follicular Dermal papilla cells (HFDPC)

• End Point

  Cell proliferation

• Reference Drugs

  Minoxidil Sulphate

• Relevance

  DPC mitotic activity

Apoptotic markers in DPCs Annexin, cell cycle in DPCs to evaluate Hair growth promotion using Human Follicular Dermal papilla cells (HFDPC), measuring Annexin and cell cycle.

• Cell Model

  Human Follicular Dermal papilla cells (HFDPC)

• End Point

  Annexin, cell cycle

• Reference Drugs

  Minoxidil Sulphate

• Relevance

  Hair growth promotion

Enhanced VEGF, KGF, IGF secretion in DPCs to evaluate Hair growth promotion using Human Follicular Dermal papilla cells (HFDPC), measuring increase in VEGF, KGF, IGF secretion.

• Cell Model

  Human Follicular Dermal papilla cells (HFDPC)

• End Point

  Increase in VEGF, KGF, IGF secretion

• Reference Drugs

  Minoxidil Sulphate

• Relevance

  Hair growth promotion

Mechanistic pathways in DPCs-MAPK, ERK, WNT pathways to evaluate Hair growth promotion using Human Follicular Dermal papilla cells (HFDPC), measuring percent modulation of markers.

• Cell Model

  Human Follicular Dermal papilla cells (HFDPC)

• End Point

  Percent modulation of markers

• Reference Drugs

  Minoxidil Sulphate

• Relevance

  Hair growth promotion

Increase in migration of DPCs to evaluate Hair growth promotion using Human Follicular Dermal papilla cells (HFDPC), measuring percent modulation of migration.

• Cell Model

  Human Follicular Dermal papilla cells (HFDPC)

• End Point

  Percent modulation of migration

• Reference Drugs

  Minoxidil Sulphate

• Relevance

  Hair growth promotion

Cytokines inhibition in DPCs to evaluate Hair anchorage/Scalp health using Human Follicular Dermal papilla cells (HFDPC), measuring Inhibition of cytokines as compared to stimulation.

• Cell Model

  Human Follicular Dermal papilla cells (HFDPC)

• End Point

  Inhibition of cytokines as compared to stimulation

• Reference Drugs

  Minoxidil Sulphate/Dexamethasone

• Relevance

  Hair anchorage/Scalp health

Protection against oxidative/UVB induced stress in DPCs to evaluate Aging associated hair loss using Human Follicular Dermal papilla cells (HFDPC), measuring restoration of cell viability.

• Cell Model

  Human Follicular Dermal papilla cells (HFDPC)

• End Point

  Restoration of cell viability

• Reference Drugs

  Minoxidil Sulphate

• Relevance

  Aging associated hair loss

Proliferation in keratinocytes to evaluate Scalp health using Keratinocytes (HaCaT), measuring increased proliferation by BrdU.

• Cell Model

  Keratinocytes (HaCaT)

• End Point

  Increased proliferation by BrdU

• Reference Drugs

  L-ascorbic acid/ EGF/ FBS

• Relevance

  Scalp health

Ex vivo hair growth assay using murine vibrissae hair follicles to evaluate Effect on hair bulb, shaft thickness and shaft length using Murine vibrissae hair follicle, measuring effect on hair bulb, shaft thickness and shaft length.

• Cell Model

  Murine vibrissae hair follicle

• End Point

  Effect on hair bulb, shaft thickness and shaft length

• Reference Drugs

  Minoxidil sulfate

• Relevance

  Effect on hair bulb, shaft thickness and shaft length

Inhibition of DPCs proliferation by BrdU incorporation in Human Follicular Dermal papilla cells (HFDPC) to study hair growth inhibition

• Cell Model

  Human Follicular Dermal papilla cells (HFDPC)

• End Point

  Inhibition of cell proliferation

• Reference Drugs

  Eflornithine

• Relevance

  Hair growth inhibition

Telogen to anagen model to evaluate Hair growth promotion using C57 BL/6 mice, measuring Hair growth score, Follicle count.

• Animal Model

  C57 BL/6 mice

• End Point

  Hair growth score, Follicle count

• Reference Drugs

  5% Minoxidil/ 10% Minoxidil

• Relevance

  Hair growth promotion

Chemotherapy induced alopecia model- Curative Model to evaluate Hair regrowth using C57 BL/6 mice, measuring hair regrowth, follicle count.

• Animal Model

  C57 BL/6 mice

• End Point

  Hair regrowth, Follicle count

• Reference Drugs

  10% Minoxidil

• Relevance

  Hair regrowth

Chemotherapy induced alopecia model- Preventive to evaluate Delay in CIA induction using C57 BL/6 mice, measuring delay in CIA induction, follicle count.

• Animal Model

  C57 BL/6 mice

• End Point

  Delay in CIA induction, Follicle count

• Reference Drugs

  10% Minoxidil

• Relevance

  Delay in CIA induction

Inhibition of hair growth using mice model in C57 BL/6 mice to study Slow hair growth rate, reduction in hair growth/ hair density.

• Animal Model

  C57 BL/6 mice

• End Point

  Slow hair growth rate, Reduction in hair growth/ hair density

• Reference Drugs

  Eflornithine

• Relevance

  Slow hair growth rate, Reduction in hair growth/ hair density