In-vitro cytotoxicity (Balb/c 3T3) neutral red uptake assay to evaluate Acute mammalian toxicity (oral) using Murine fibroblast (Balb/C 3T3), measuring Neutral red uptake.

• Cell Model

  Murine fibroblast (Balb/C 3T3)

• End Point

  Neutral red uptake

• Reference Drugs

  Sodium Lauryl Sulphate

• Relevance

  Acute mammalian toxicity (oral)

In-vitro CFU-GM assay to evaluate Hematotoxicity using Murine Bone marrow cells, measuring Colony Counting.

• Cell Model

  Murine Bone marrow cells

• End Point

  Colony Counting

• Reference Drugs

  Paclitaxel

• Relevance

  Hematotoxicity

In-vitro skin irritation assay (Reconstructed Human Epidermis) to evaluate dermal safety using reconstructed Human Epidermis, measuring viability by MTT.

• Cell Model

  Reconstructed Human Epidermis

• End Point

  Viability by MTT

• Reference Drugs

  SDS

• Relevance

  Dermal safety

In vitro eye irritation assay (HET-CAM) to evaluate ocular safety using Chorioallantoic membrane of fertilized chicken egg, measuring Irritation score.

• Cell Model

  Chorioallantoic membrane of fertilized chicken egg

• End Point

  Irritation score

• Reference Drugs

  SDS

• Relevance

  Ocular safety

Determination MTD to select safe dose for efficacy studies in Swiss Albino Mice to study relevant biological pathways.

• Animal Model

  Swiss Albino Mice

• End Point

  % Body Weight Change, Mortality, Hematology & Biochemistry

• Reference Drugs

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