This assay quantifies analyte of interest in different matrix samples of in-vivo and in-vitro studies using suitable chromatography techniques like HPLC, LCMS, GC.

• Model

  Using suitable chromatography techniques like HPLC, LCMS, GC

• End Point

  Robust bioanalytical method developed to estimate analyte concentration in in-vitro and in-vivo samples precisely and accurately.

• Relevance

  To quantify analyte of interest in different matrix samples of in-vivo and in-vitro studies.

This assay evaluates the pharmacokinetic parameters of analyte required to know drug absorption, distribution, metabolism and excretion profile.

• Animal Model

  Rodent

• End Point

  Pharmacokinetic parameters like AUC, Cmax, Tmax, Vd, Cl etc.

• Relevance

  To evaluate the pharmacokinetic parameters of analyte required to know drug absorption,distribution,metabolism and excretion profile.

This assay identifies the bioavailability of drug/analyte using suitable animal model.

• Animal Model

  Rodents/Rabbit

• End Point

  % Bioavailability will be evaluated

• Relevance

  To identify the bioavailability of drug/analyte

This assay identifies the biodistribution of drug/analyte in different tissues using suitable animal model.

• Animal Model

  Rodents/Rabbit

• End Point

  Drug distribution in different tissue will be evaluated

• Relevance

  To identify the biodistribution of drug/analyte in different tissues

This assay identifies the drug metabolite formation or clearance of analyte using suitable animal model.

• Animal Model

  Rodents

• End Point

  Drug metabolic identified and drug clearance will be evaluated

• Relevance

  To identify the drug metabolite and drug clearance

This assay identifies drug to drug interaction when taken simultaneously using suitable animal model.

• Animal Model

  Rodents

• End Point

  Drug inhibition and drug induction potential

• Relevance

  To identify drug to drug interaction when taken simultaneously

This assay identifies drug permeability through cell based and non-cell based models.

• Cell Model

  CaCo-2 cells, MDCK, PAMPA (non-cell based)

• End Point

  Drug permeability properties.

• Relevance

  To identify in-vitro drug permeability

This assay identifies drug to drug interaction when taken simultaneously using suitable chromatography techniques like HPLC, LCMS, GC.

• Model

  Using suitable chromatography techniques like HPLC, LCMS, GC, CYP Inhibition kits

• End Point

  Drug inhibition potential will be evaluated.

• Relevance

  To identify drug to drug interaction when taken simultaneously

This assay identifies binding of drug to plasma protein using ultracentrifugation, ultrafiltration or rapid equilibrium dialysis method and suitable chromatography techniques like HPLC, LCMS, GC.

• Model

  Using Ultracentrifugation, Ultrafiltration or Rapid equilibrium dialysis method and suitable chromatography techniques like HPLC, LCMS, GC

• End Point

  Drug plasma protein binding will be evaluated, bound and unbound fraction of drug will be evaluated.

• Relevance

  To identify binding of drug to plasma protein

This assay identifies drug permeability through animal skin model or or artificial membranes using franz diffusion cell and suitable chromatography techniques like HPLC, LCMS, GC.

• Animal Model

  Animal skin model or Artificial membrane

• End Point

  Drug permeability potential will be evaluated.

• Relevance

  To identify drug permeability potential through animal skin

This assay validates analytical methods for accurate analyte measurement.

• Model

  Using suitable chromatography techniques like HPLC, LCMS, GC

• End Point

  Selectivity, Specificity, Matrix effect, Calibration curve and range, Accuracy and precision, Carry-over, Dilution integrity, Stability

• Relevance

  Confirms that the method used for the analysis is reliable, reproducible and fit for its intended purpose

This assay determines systemic exposure of compound during toxicology assessment using suitable animal model.

• Animal Model

  Rodents/Rat

• End Point

  Quantification of analyte/(s) in biological matrix

• Relevance

  Determine systemic exposure of compound during toxicology assessment.